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. 2006 May 10;26(19):5037–5048. doi: 10.1523/JNEUROSCI.0715-06.2006

Figure 1.

Figure 1.

OLP specification from Nkx2.2-expressing progenitors correlates with the end of neuronal production in the ventral spinal cord. A–F, Immunodetection of Olig2, Nkx2.2, and O4 on transverse sections of E4 (A, C) and E6 (B, D, E, F) spinal cords. A, B, E, Double immunodetection of Olig2 (red) and Nkx2.2 (green) showing the mutually exclusive spatial Olig2+ pMN domain and Nkx2.2+ p3 domain at E4 (A) and the formation of the Olig2+/Nkx2.2+ p* domain at E6 (B). E, Higher magnification of B, showing the coexpression of Olig2 and Nkx2.2 in the nascent p* domain, whereas the ventral-most Nkx2.2+ cells of the p3 domain do not express Olig2. C, D, F, Double immunodetection of Olig2 (red) and O4 (green). O4+ cells were never found in E4 spinal cord (C). Note the detection of O4 expression in dorsal root ganglia (A, arrowheads). At E6, O4+ OLP are detected in the ventral-most region of the neuroepithelium, adjacent to the floor plate. F, Higher magnification of the ventral neuroepithelium showing the coexistence of Olig2+/O4+ and Olig2−/O4+ cells in the p* and p3 domains, respectively. Note that, at this stage, Olig2−/O4+ cells were already migrating away from the neuroepithelium (F, arrowhead). G, H, Ventral neuroepithelial explants isolated from E4 (G) and E7 (H) embryos and maintained 2 d in culture. Detection of sim1 by in situ hybridization shows that V3 ventral interneurons differentiate from E4 (G) but not from E7 ventral neural progenitors (H). I–L, Detection of ebf2 and neuroM on transverse vibratome sections of E4 (I, K) and E6 (J, L) spinal cords. At E4, both neuronal markers are detected all along the dorsoventral axis of the spinal cord in cells lining the neuroepithelium (I, K), whereas at E6, ebf2 and neuroM expressions have disappeared in the region adjacent to the ventral-most domain of the neuroepithelium (J, L, brackets).