Figure 3.

Immunohistochemical evidence for activity-dependent cycling of GluR2-containing AMPARs in cultured retinal neurons. A, Cultured retinal neurons were incubated with an antibody recognizing the extracellular epitope of GluR2 for 30 min and washed thoroughly. Cells were fixed immediately (0 min; left) or were incubated in complete media for 45 min before fixation and secondary antibody application (right). In a control group (top), surface staining was decreased, whereas there was little internalization in cells treated with AMPA overnight (bottom). Scale bar, 5 μm. B, Summary of the results from five experiments in the control group and four experiments in the AMPA-treated group. ∗∗p < 0.01. Error bars represent SEM.