Figure 6.

Distribution of types 1, 2, and 3 RyRs. A, Representative immunofluorescent images from control (preimmune serum) and types 1 and 2 RyRs. B, Representative immunofluorescent images from control (purified IgG) and type 3 RyR. For both A and B, each panel is an optical section (∼0.65 μm thick) through the middle z plane of the stack of images for the terminal. The left column shows the deconvolved images; the tracing around the perimeter of the terminal was drawn by eye on these images. The right column shows the corresponding images after correction as outlined in Materials and Methods and Figure 1.