Figure 1.

Pro-NGF elicits loss of BF neurons in culture. BF neurons were grown for 5 d and treated overnight with the following: vehicle (imidazole), mature NGF (100 ng/ml), pro-NGF (1 ng/ml), pro-NGF (1 ng/ml) in the presence of blocking antibodies to NGF (Sigma; which also block pro-NGF; diluted in the medium 1:500) or p75 (9651 (Huber and Chao, 1995; Friedman, 2000); diluted 1:500 in the medium), or the antibodies alone. Data are expressed as mean percentage of control ± SEM in triplicate cultures from five independent experiments (n = 15). The asterisk indicates a value different from control at p < 0.05. Veh, Vehicle; mNGF, mature NGF.