Table 2.

Pro-NGF induces apoptosis of P-Trk+ but not P-Akt+ neurons

	Vehicle	BDNF (15 min)	Pro-NGF (30 min)	BDNF (15 min) + Pro-NGF (30 min)
P-Trk/TrkBa
% Healthy	65/94	97/97	54/75	72/72
% Apoptotic	0/6	0/3	8/25	28/28
P-Akt /P-Trkb
% Healthy	93/93	92/92	68/68	56/56
% Apoptotic	0/7	0/8	0/32	0/44
P-Akt /CC3c	No double labeling (see Fig. 9C); 100% of the P-Akt+ cells were healthy and lacked cleaved caspase-3 (CC3) staining, and 100% of the CC3+ were apoptotic and lacked P-Akt labeling.

BF neurons were grown on slide wells for 5 d and treated with BDNF and/or pro-NGF as indicated. Neurons were double labeled with the antibodies indicated in column 1 and scored as healthy or apoptotic based on Hoechst nuclear labeling. Numbers indicate the percentage of neurons labeled with both antibodies out of the percentage of neurons with only the second antibody that were either healthy or apoptotic.

^aPercentage of TrkB + neurons that also were labeled for P-Trk that were healthy or apoptotic with the indicated treatments (e.g., in vehicle-treated cultures, 94% of the neurons with TrkB were healthy, 65% of those had P-Trk as a result of the neurons being grown with BDNF; 6% of the TrkB-positive neurons were apoptotic, none had P-Trk.) Note that with BDNF + pro-NGF all the apoptotic neurons with TrkB also had P-Trk indicative of receptor activation.

^bPercentage of healthy or apoptotic P-Trk + neurons that were also labeled for P-Akt in the different treatment conditions. Note that none of the apoptotic neurons were labeled for P-Akt (Fig. 9).

^CDouble labeling was performed for cleaved caspase-3 and P-Akt; no overlap was observed. Cells were counted in 10 random fields in duplicate wells from three independent experiments.