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. 2006 Jul 19;26(29):7693–7706. doi: 10.1523/JNEUROSCI.0522-06.2006

Figure 5.

Figure 5.

Unaltered GKAP localization after combined treatment with SAP97 RNAi and 2-bromopalmitate. A, Cotransfection of SAP97 or PSD-95 RNAi vector with SAP97-EGFP in COS7 cells. Selective suppression of SAP97-EGFP was observed in cells expressing SAP97 siRNA. DIC, Differential interference contrast. B, Knockdown of endogenous SAP97 expression by SAP97 RNAi. The SAP97 RNAi vector was cotransfected with GFP. C, Enhanced reduction in synaptic MAGUK proteins by combined treatment of primary neurons with SAP97 RNAi and 2-bromopalmitate. D, Preserved synaptic clustering of GKAP after combined treatment with SAP97 RNAi and 2-bromopalmitate. E, Fluorescence quantitation of synaptic anti-MAGUK and anti-GKAP staining in neurons treated with SAP97 RNAi and/or 2-bromopalmitate. The amount of GKAP in synapses did not change after combined treatment with SAP97 RNAi and 2-bromopalmitate. Error bars indicate SEM. Scale bars: A, 150 μm; B–D, 10 μm. 2-Br-Pal, 2-Bromopalmitate.