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. 2006 Jul 19;26(29):7693–7706. doi: 10.1523/JNEUROSCI.0522-06.2006

Figure 9.

Figure 9.

Activity-dependent redistribution of multiple PSD proteins and their altered dynamics. A, Enhancement of spike firing by treatment of neurons with bicuculline/4-AP (from 5.17/min to 27.53/min; average of 15 min period). B, Rapid remodeling of PSD proteins after application of bicuculline/4-AP. Enhancement of synaptic activity by bicucullin/4-AP treatment induced the increase in GKAP, decrease in Shank and PSD-Zip45, and transient increase in actin at the postsynaptic sites. Scale bar, 2 μm. C, Quantitation of fluorescence change after bicuculline/4-AP treatment. Previous incubation of cells with the actin-stabilizing reagent jasplakinolide completely suppressed redistribution of GKAP, Shank, and PSD-Zip45. D, Suppression of scaffolding protein dynamics by bicuculline/4-AP treatment. FRAP analysis of neurons exposed to bicuculline/4-AP revealed significant suppression of fluorescence recovery of EYFP-GKAP and EGFP-PSD-Zip45. When recovery curves were normalized for the amount of exchangeable fraction within 300 s, an altered time constant of GKAP turnover after bicuculline/4-AP treatment was evident. Error bars indicate SEM. bic, bicuculline.