Skip to main content
. 2006 Jul 19;26(29):7619–7628. doi: 10.1523/JNEUROSCI.0855-06.2006

Figure 4.

Figure 4.

Retinal projections are required for OPC induction at the third ventricle midline. One (A) or both (B) eyes were removed from embryos at stage 21 (E3), and the animals were allowed to develop until stage 31 (E7). In control animals, many O4+ OPCs were present at the third ventricle floor (arrow) at E7 (C) but were absent or greatly reduced (arrow) in eyeless animals (D). E, O4+ OPCs in the lateral region of the third ventricle were not affected by the removal of both eyes. F, G, The residual OPCs in the floor of the third ventricle were located more caudally in eyeless animals. H, I, Explants derived from the floor of the third ventricle of eyeless animals at stage 29 (E6) demonstrated a reduced ability to generate OPCs after 48 h in vitro. J, Quantification of O4+ cell numbers demonstrated a significant reduction of O4+ cells in the floor of the third ventricle in eyeless animals. K, Comparison of OPC development in control and eyeless animals. Although all control explants contained >10 OPCs, 50% of explants from eyeless animals had <10 OPCs. Data represent the proportion of explants containing different cell numbers from three different preparations. Scale bars: C–E, 25 μm; F–I, 50 μm.