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. 2006 Jul 19;26(29):7619–7628. doi: 10.1523/JNEUROSCI.0855-06.2006

Figure 5.

Figure 5.

Retinal induction of OPCs is dependent on hedgehog signaling. A–F, Stage 24 (E4) retinal explant conditioned medium induces OPCs in stage 24 (E4) dorsal spinal cord cells after 4 d. A, B, Control cultures containing few OPCs. C, D, Retinal CM induces significant numbers of OPCs. E, F, This induction is inhibited by treatment with cyclopamine. G, Quantification of O4+ cells in cultures treated with Shh function-blocking mAb 5E1 shows that the induction is inhibited in a dose-dependent manner. Error bars represent SD (3 different preparations). H, I, In cocultures of retinal and dorsal spinal cord explants, the induction of OPCs is reduced significantly by cyclopamine treatment. J, Quantification of O4+ cells in control cocultures and cocultures treated with cyclopamine. K, L, Likewise, the inductive capacity of retinal neurites in the Campenot chambers is blocked by the addition of cyclopamine to the lateral chamber. M, Quantification of O4+ cells in Campenot chambers. Scale bars (in D, K), 50 μm.