Figure 1.
The TACE gene is expressed in both isolated hypothalamic glial cells and the intact rat hypothalamus. A, Detection by RT-PCR of TACE mRNA in cultures of purified astrocytes and ependymoglial cells (tanycytes) and in the MBH of immature female rats. MM, Molecular marker. B, Cellular localization of TACE mRNA in the hypothalamus of prepubertal (30-d-old) female rats as detected by in situ hybridization using a [35S]UTP-labeled cRNA probe. Although TACE mRNA-containing cells are present throughout the hypothalamus, including ependymal cells lining the wall of the third ventricle (3V; arrowheads), the most intense hybridization signal is observed in the ME (arrows). Scale bar, 200 μm. C, Higher-magnification image illustrating the abundance of TACE mRNA in glial cells of the ME. Scale bar, 50 μm.