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Colocalization of AADC (red) and TH (green) in the vPAG. Nearly all of the TH-ir neurons contained AADC (visualized as yellow color over green neurons; small white arrows); a rare TH-ir cell that did not contain AADC is indicated by the large white arrow. The AADC-ir neurons that were not TH-ir (arrowhead; red cells) are presumably serotoninergic. Scale bar, 100 μm.
