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Sources of afferents to the vPAG. CTB injected into the vPAG retrogradely labels neurons (red) in the orexin-ir field (green) in the lateral hypothalamus. Arrows point to double-labeled neurons (yellow). After a similar injection, retrogradely labeled neurons (brown) are seen in the medial prefrontal cortex (B) and in the LC (C). Black fos staining is seen in both B and C in this sleeping animal. MPFC, Medial prefrontal cortex. Scale bar: (in C) A, 100 μm; B, C, 250 μm.
