Figure 1.

Juxtacellular labeling and extracellular recording technique. Left, Unit discharge of a pontine respiratory neuron before, during, and after juxtacellular labeling (from top to bottom). Note entrainment of unit discharge during juxtacellular current injection and the recovery of spontaneous discharge after injection. Right, Schematic drawing showing the position of the micropipette juxtaposed with the neuron being recorded. The tip of the micropipette presumably “touched” the neuron membrane without penetration during current injection.