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. 2006 Aug 16;26(33):8502–8511. doi: 10.1523/JNEUROSCI.1758-06.2006

Figure 6.

Figure 6.

Effects of latrunculin and nocodazole on transitions between the compartments and dwell times at synapses. A, Processing of single GlyR-QD reconstructed trajectories: example in latrunculin conditions. x-axis, Recording time (in seconds). y-axis, QD state: 1, synaptic; 0, extrasynaptic; −1, nonemitting. A1, State of the GlyR-QD plotted as a function of time from a 13 Hz recording. A2, State of the GlyR-QD after blink removal and time reallocation. A3, Filtering of GlyR-QD transitions between the synaptic and extrasynaptic locations. The red line (y = 0.5) corresponds to the criteria chosen to remove artificial transitions attributable to pixelization of the FM image. A4, Resulting location of the GlyR-QD. The black and purple stars indicate nonemitting events in which zero or one transition is estimated, respectively. The red circles indicate an example in which filtering attenuates the number transitions. The dwell times and number of transitions are determined after this processing. B, Increased number of transitions between the synaptic and extrasynaptic compartments after Lat (red) and Nz (green) treatments compared with control conditions (Ctr) (blue). C, Decreased dwell time at synapses after latrunculin and nocodazole treatment. D, Latrunculin (D1) and nocodazole (D2) effects on transitions between the synaptic and extrasynaptic compartments (trans) (dashed line) and dwell time at synapses (d.t.) (dotted line) compared with fluorescence intensity of GlyR synaptic clusters (IF) (full line). The intermediate time for transitions and dwell time at synapses corresponds to the average of 9 recordings in latrunculin and 12 in nocodazole conditions acquired 4–10 min after the addition of the drug. Note that drug effects on single GlyR-QD dynamics and GlyR synaptic levels are rapid and concomitant. All data are from two independent cultures. Values are mean ± SEM. Statistical analyses: *p < 5 × 10−2, **p < 10−2, ***p < 10−3; dwell times and transitions: Mann–Whitney test; fluorescence intensity: ANOVA, PLSD test.