Figure 1.

Cre recombination mediated by the GCE transgene is inducible by OHT in vivo. OHT (33 mg/kg) or sunflower oil vehicle were injected once or twice a day (single or double green arrow) in GCE;R26R double transgenic mice. Analysis was at the time points indicated by the red arrows. All sections show X-gal staining in line 505. A–D, Whole-mount X-gal staining in GCE⁺/tamoxifen⁺ (A, B) or GCE⁺/tamoxifen⁻ (C, D). E–L, X-gal-stained sections from GCE⁺/tamoxifen⁺ (E, F, I–L) or GCE⁺/tamoxifen⁻ (G, H). The white arrows in E point to X-gal staining in SVZ and DG. I–L, x-gal/GFAP double staining in DG (I, K) and cerebellum (J, L). K and L show high magnification views of the DG and the Purkinje layer, respectively. The arrows point to GFAP-stained fibers with X-gal⁺ cell bodies. M, Whole-mount specimens stained for X-gal at different times after two OHT injections at P5. N–Q, Sections from GCE mice treated with sunflower oil vehicle (N) or with two OHT injections at P5 (O–Q) and analyzed at P35. a, Analysis; ctx, cerebral cortex; th, thalamus; sc, superior colliculus; ic, inferior colliculus; pn, pons; dg, dentate gyrus; h, hilus; igl, internal granular layer; ml, molecular layer. Scale bars: E–H, 200 μm; I, N, P, Q, 12.5 μm; J, O, 25 μm; K, L, 5 μm.