Figure 8.

Overexpression of a dominant-negative version of Apl I in the sensory neuron blocks ITF. A, Sample traces from experiments in which the ITF induction protocol was applied to a control culture and to a culture in which the sensory neuron expressed a dominant-negative (DN) form of Apl I. Both cultures show STF after a 5 min exposure to 5-HT, but only the control shows ITF at 30 and 60 min. Calibration: 20 mV, 25 ms. B, ITF was blocked in cocultures that expressed dominant-negative Apl I (p < 0.001 at 30 min, p < 0.01 at 60 min; two-way ANOVA). The controls in B consisted of cultures in which the sensory neuron was not injected with any plasmid or cultures in which the sensory neuron expressed a wild-type eGFP-Apl I. The two types of control were not different from each other (p > 0.05) and were therefore pooled.