Figure 2.

L5 SNL induces Src-family activation in spinal microglia but not in neurons or astrocytes. Immunohistochemical colocalization of green reaction product for p-Src family (A, D, G, J) and red product for MAP2 (B), NeuN (E), GFAP (H), or OX42 (K) in the ipsilateral dorsal horn at day 7 after nerve injury. A–F, Double staining of p-Src family with neuronal markers MAP2 and NeuN shows no colocalization. G–I, Double labeling of p-Src family with GFAP, an astrocyte marker, indicates that Src family is not activated in astrocytes. J–L, Double staining of p-Src family with OX42, a microglia marker, reveals a heavy colocalization in the dorsal horn. M–O, Double immunofluorescence shows that p-Src family (green) is colocalized with OX42 (red) in the superficial dorsal horn (laminas I–III) at days 1, 3, and 14 after nerve injury (M, N, and O, respectively). Two single-stained images were merged. Scale bar, 20 μm.