L5 SNL induces the activation of SFKs, such as Src, Lck, and Lyn, in spinal microglia. A, p-Src immunostaining in the ipsilateral (Ipsi) dorsal horn at day 7 after surgery. Contra, Contralateral. B–D, Double staining indicates a complete colocalization (D) between p-Src (B) and p-Src family (C) in the ipsilateral dorsal horn at day 7 after surgery. E, F, p-Lck (E) and p-Lyn (F) immunostaining in the ipsilateral dorsal horn at day 7. G–L, Double labeling for OX42-IR (H, K) and p-Lck-IR (G) or p-Lyn (J) in the ipsilateral dorsal horn at day 7. Scale bars: (in I) A, E, F, 100 μm; (in L) G–L, 20 μm.