Figure 4.

Coimmunoprecipitation of C99 and sorLA. C99-myc was pulled down by myc-beads and probed for sorLA by LA–sorLA antibody (lane 1). N2a cells transfected with C99-myc (lane 2) or transfected with sorLA only (lane 3), showed no nonspecific pull-down. Specific coimmunoprecipitation of sorLA was also observed when immunoprecipitating transfected C99ΔGYENPTY-myc mutant construct (lane 4). For all experiments, specific bands for C99-myc stained with 13G8 antibody or sorLA were shown from cell lysates and pull-down.