Figure 10.

Protective effects of delayed NAm treatment on inflammation, demyelination, and axonal loss in the EAE model. A, B, Representative images showing the effects of delayed NAm treatment on infiltration, demyelination, and axonal loss. Transverse sections from wild-type EAE animals with delayed NAm treatment at 8 weeks p.i. were stained with Hoechst 33258 and antibodies against MBP and NF (A). Higher magnification of the merged image is shown in B. The arrowheads indicate several preserved axons in the demyelinated lesions. Scale bars: A, 50 μm; B, 10 μm. C–E, Quantification of the average areas of infiltration (p = 0.65) (C) or demyelination (p = 0.14) (D) showed no significant difference, but the average number of NF+/MBP− fibers in demyelinated areas was increased in animals with delayed treatment with NAm (*p < 0.05; Student's t test) (E). Error bars indicate SEM.