Figure 3.

Wld^s expression reduces axon loss at 2 weeks p.i. A–C, Representative images of transverse sections from the lumbar spinal cords of EAE-induced wild-type or Wld^s mice at 2 weeks p.i. Images of sections stained with Hoechst 33258/anti-MBP/NF (A, B) show a similar extent of infiltration, but significantly more NF+ fibers in the infiltrated and demyelinated areas from Wld^s EAE mice compared with wild-type EAE mice. Images in B are enlarged from the indicated areas in A. The arrowheads indicate several preserved axons in the lesion areas. Low magnification images of Hoechst 33258 staining indicating similar extents of infiltration in both wild-type and Wld^s mice induced with EAE (C). Scale bars: A, C, 100 μm; B, 10 μm. D–F, Quantification of the average areas of infiltration (p = 0.97) (D) and demyelination (p = 0.25) (E) per section, which showed no significant difference, and the average numbers of NF+/MBP− fibers in demyelinated areas, which was significantly increased in Wld^s mice compared with wild type (**p < 0.001; Student's t test) (F). Error bars indicate SEM.