Figure 5.

The effects of Wld^s on infiltration of immune cells and cell death in the EAE model. Sagittal sections from 2 weeks p.i. EAE-induced wild-type or Wld^s mice were stained with Hoechst 33258 and antibodies against CD4 (A). Representative images are shown in A. The panel shown in B is an enlargement of the selected area of merged image. Scale bars: A, 25 μm; enlarged panel, 10 μm. Quantification of the average number of infiltrated CD4+ (C) cells (numbers per section) from six sections per animals, eight animals per group, showing no significant differences in these groups (p = 0.97; Student's t test). D, Representative images of TUNEL (red in bottom panel) costained with Hoechst 33258 (white in top panel and blue in bottom panel) in the lesion areas of the cervical spinal cord transverse sections of wild-type (left panels) or Wld^s (right panels) mice at 2 weeks p.i. Scale bars, 15 μm. E, Quantification of TUNEL-positive cells in EAE-induced wild-type and Wld^s mice (10 sections per animal from 8 animals in each group) at 2 weeks p.i., showing no significant difference in these groups (p = 0.93; Student's t test). Error bars indicate SEM.