Figure 9.

Protective effects of delayed NAm treatment on behavioral defects in the EAE model. A, Behavioral scores (mean ± SEM) of EAE in untreated (n = 14) or delayed treated (DT) (n = 12) groups. Differences between these groups were significant as determined by two-tailed Student's t test (p < 0.01 from 13 d p.i.). The differences seen before 10 d p.i. are attributable to the death of three mice in the untreated group when they showed clear behavioral deficits. B, NAD levels (mean ± SEM) of cervical spinal cords from untreated or delayed treated groups. At both 2 and 4 weeks p.i., the NAD levels in the treated groups were significantly higher than those in untreated animals (*p < 0.05; **p < 0.001; Student's t test). The arrows indicate the starting point of delayed treatment in both A and B.