Figure 1.

Choline uptake and choline-induced current in hCHT-expressing oocytes. A, Uptake assays were performed with 10 μm choline containing 1% [³H]choline and measured over a period of 30 min in both hCHT WT-expressing and LV-expressing oocytes with and without 10 min preincubation of the hCHT-specific inhibitor HC-3 at 1 μm (n = 5–8). B, The choline-induced I–V curves were generated by brief exposure to 10 μm choline at various membrane potentials (n = 5). Baseline current in the absence of choline was subtracted from the choline-induced current. Inset, Choline at 10 μm induced a significant current when the oocyte was held at −60 mV. Note that the holding currents in WT- and LV-expressing oocyte are shifted compared with control oocyte.