Figure 8.

Presence of low-level NF-H and NF-M in the parallel fibers of cerebellar granule cells in wild-type (wt) mice. A–D, Immunocytochemistry with SMI 33 and SMI 31 antibodies confirms the presence of low-level NF-H and NF-M in the parallel fibers of cerebellar granule cells in wild-type mice (A, C) and their absence in hm-dko mice (B, D). E–H, Anti-NF-H antibody N52 detects NF-H in the parallel fiber (E) and demonstrates its colocalization with α-internexin (F–H). A and B were stained with SMI33, and C and D were stained with SMI31. E and F were stained with N52, and G was stained with α-internexin antibody. H is the merge of F and G. ML, Molecular layer; PC, Purkinje cells; GC, granule cells. The arrows point to parallel fibers. Scale bars: A, B, 160 μm; C–H, 40 μm.