Figure 1.
Light regulates AANAT apparent Km and Vmax in the chicken retina. Apparent Km and Vmax of AANAT were measured in chicken retinas dissected at ZT18 in darkness or after 5–60 min of light exposure. Daytime values were obtained at ZT6. Animals were injected intraocularly with 10 μl of water or 2.5 mm lactacystin. Km and Vmax data are presented in A and B, respectively. For Km measurements, two-way ANOVA indicates a significant effect of light (p < 0.001) but not of lactacystin (p = 0.407). For Vmax measurements, there were significant effects of both light (p < 0.05) and lactacystin (p < 0.01) and a significant interaction of light and lactacystin (p = 0.05). Values are means ± SEM (n = 4).