Figure 10.

PKA and 8-Br-cAMP favor the formation of AANAT/14-3-3 complex. Proteins from dark-adapted nighttime retinas were incubated in the presence of 2 mm ATP, 15 mm MgCl₂ plus 15 U of catalytic subunit of PKA (A), or 100 μm 8-Br-cAMP (B) and separated by FPLC on a Superdex 75 10/300 GL column. Representative FPLC profiles, peak square data, and statistical evaluation are shown for each treatment. The thin arrows on FPLC profiles indicate the location of molecular weight standards; from left to right: 128 kDa, 44 kDa, and 17 kDa. *p < 0.05, Student–Newman–Keuls test following one-way RM-ANOVA, p < 0.05. Values are means ± SEM (n = 3).