Table 1.

Total cellular DA and DOPAC contents in PC12 cells before and after l-DOPA treatment

	Untreated			l-DOPA			l-DOPA/untreated
	DA	DOPAC	DA/DOPAC	DA	DOPAC	DA/DOPAC	DA	DOPAC
Control (EGFP)	680 ± 120	13.3 ± 2.5	40 ± 4	8330 ± 1380	960 ± 70	8.7 ± 1.4	12	72
WT synuclein (Σm1)	150 ± 40*	4.5 ± 0.3*	34 ± 7	2540 ± 340	490 ± 145	8.7 ± 2.4	17	110
WT synuclein (Σp1)	300 ± 60	11.4 ± 1.7	33 ± 10	15060 ± 1040	1860 ± 380	9.7 ± 1.5	51	164
A30P synuclein (Am9)	650 ± 80	9.3 ± 0.5	51 ± 11	13360 ± 730	690 ± 60	19.5 ± 0.8*	21	74
A53T synuclein (11p2)	760 ± 30	13.2 ± 0.5	58 ± 4	29450 ± 3050*	1870 ± 210	15.8 ± 0.6*	39	142

PC12 cells were treated with 100 μm l-DOPA for 1 hr at 37°C before harvesting and fixation (see Materials and Methods). Total intracellular levels of DA and DOPAC were determined by HPLC with electrochemical detection and normalized to protein concentration in each sample. Data are shown as mean ± SEM of metabolite concentrations in femtomoles per microgram of protein from three to six samples (culture dishes) for each cell line.

*p < 0.05, different from EGFP cells (Kruskal–Wallis test).