Figure 1.

PAD4 in normal white matter and MS normal-appearing white matter. A, The differential distribution of PAD (1–4) proteins in MS samples was confirmed by fractionation of brain extracts into membrane (A + B), microsomal (C), and nuclear (D) fractions. The amount of total PAD protein was measured, and the results are presented as a bar graph of values in MS samples relative to normal controls. B, Anti-PAD4 Western blot of nuclear fraction from cortical white matter further supported the increased levels of PAD4 in the nuclear fraction of MS sample compared with the levels in normal controls. The nuclear fractions used in the PAD4 Western blot were as follows: Normal 1, 2, and 3 are HSB 3236, 3276, and 3322; MS 4–9 are HSB 3502, 2429, 3522, 3509, 2800, and 2485, respectively. These individuals are described further in supplemental Table 1 (available at www.jneurosci.org as supplemental material).