Figure 4.
Nuclear PAD4 protein and TNF-α in ND4 brain. A, Western blot with an anti-PAD4 polyclonal antibody of nuclear fractions prepared from 2-, 3-, and 6-month-old ND4 and normal littermates. Histone H3 immunoreactivity was used as a loading control. B, Nuclear PAD4 activity was measured by determining the H3cit/H3 ratio in ND4 brains relative to normal littermates from 2–8 months ± SD. C, TNF-α Western blot of whole-brain protein homogenates from normal and ND4 littermates at 2, 3, 6, and 8 months of age. Histone H3 immunoreactivity was used as a loading control.