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. 2006 Nov 1;26(44):11423–11431. doi: 10.1523/JNEUROSCI.3600-06.2006

Figure 6.

Figure 6.

Usp14LF associates with proteasomes and restores the levels of ubiquitin in axJ mice. A, The left panel shows the immunoblot of proteasome fractions from the brains of wild-type (wt), axJ, and axJ Tg mice probed with polyclonal Usp14 antisera. The blots also were probed for the 19S proteasomal component Rpt4 as a positive control for proteasomes. The right panel shows catalytically active DUBs from neuronal proteasome fractions from wt, axJ, and axJ Tg mice that were labeled with HA-Ub-VME and immunoblotted with the anti-HA mAb 12CA5. The identities of the known DUBs are indicated at the right. B, Immunoblot analysis of hippocampal proteasome fractions from wild-type mice and wild-type mice containing the Usp14LF transgene (wt Tg) probed for Usp14 and the control 19S proteasomal component Rpt1 (left panel) and active DUBs labeled with HA-Ub-VME and immunoblotted with the anti-HA mAb 12CA5 (right panel). Graphs depict quantitation of the blots. C, Immunoblot analysis of hippocampal extracts measuring levels of conjugated and monomeric ubiquitin in wild-type, axJ, and axJ Tg mice. As loading controls the blots were reprobed with a monoclonal antibody to β-actin. Error bars indicate SE.