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Absence of TNF-α does not influence gliosis. Immunohistochemistry on lumbar spinal cord for Mac-2 (A–E) and GFAP (F–J) did not reveal any apparent differences in gliosis in TNF-α−/−; SOD1G93A mice (C, E, H, J) compared with TNF-α+/+; SOD1G93A mice (B, D, G, I) at early symptomatic (B,C and G, H) or end-stage (D,E and I,J) of disease. Scale bars, 50 μm. n = 5–7 mice per group.
