Figure 6.

CRF immunoreactivity in the lateral BNST. Ethanol dependence was induced by intermittent exposure to ethanol vapors for 4 weeks, and animals were killed after 2 h of withdrawal. Brains were removed and CRF expression was measured in the lateral BNST using immunohistochemical techniques. The data are expressed as the relative density of CRF immunoreactivity (n = 3 animals). A, CRF immunoreactivity was measured from five serial sections throughout the lateral BNST. From bregma, these sections were −0.20, −0.26, −0.30, −0.40, and −0.80 mm. Although there was a trend toward a decrease in dependent animals, there was no significant change in CRF immunoreactivity in the BNST in dependent animals compared with control. B, Representative micrographs (20×) from the lateral BNST (bregma −0.30 mm) demonstrating CRF staining within this brain region. Error bars indicate SEM.