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PADRENs form a spacing pattern. PADRENs in a 17 DIV hippocampal neuron. A, Immunostaining before application of detergent (− det) using the mouse 5F9 anti-MAP2 antibody. B, Immunostaining after application of detergent (+ det) using rabbit polyclonal anti-MAP2 antibodies. C, Merge. Scale bar, 30 μm.
