Figure 2.

Quantification of arrestin in mouse retinas. A, Three wild-type retina extracts containing 0.25 pmol of rhodopsin were analyzed by Western blotting along with five samples containing recombinant mouse arrestin standards premixed with aliquots of retina extract from the arrestin knock-out mouse also containing 0.25 pmol of rhodopsin. B, The calibration curve was obtained by plotting the amount of fluorescent signal in each band against the amount of arrestin in the standard (filled circles). The amounts of arrestin in the retina samples (open circles) were determined to be 0.185, 0.211, and 0.222 pmol. The data are taken from one of two independent experiments, with the total amount of analyzed retina samples being six.