Table 3.
Frequency of microencephaly/hydrocephalus in CreERT2 embryos (line 1 and line 4) after indicated treatment at E10.5
| Mouse line | Treatment at E10.5 | Microencephaly/hydrocephalus | n |
|---|---|---|---|
| CreERT2 line 1+/− | 2 mg of tamoxifen | 77% | 22 |
| CreERT2 line 1+/− | Vehicle | 0% | 40 |
| CreERT2 line 4+/− | 8 mg of tamoxifen | 83% | 12 |
| CreERT2 line 4+/− | 2 mg of tamoxifen | 0% | 3 |
| CreERT2 line 4+/− | Vehicle | 0% | 6 |
| WT | 8 mg of tamoxifen | 0% | 8 |
| WT | 2 mg of tamoxifen | 0% | 40 |
| WT | Vehicle | 0% | 48 |
Microencephaly was determined by gross observation at E19.5. The presence of hydrocephalus was verified by transillumination of whole brains or by histological sections. WT, Wild type.