Figure 4.
A–C′, Line drawings showing distribution of Fos single-labeled and Fos plus GAD double-labeled neurons located in counting grids for the rostral MnPN and vlPOA sites, including the vlPOA cluster and extended vlPOA, from dark-sleeping (A, A’), light-sleeping (B, B’), and light sleep-deprived (C, C’) rats. D, Examples of Fos single-labeled (filled arrowhead), GAD single-labeled (open arrow), and Fos plus GAD double-labeled (filled arrow) neurons in the MnPN of a light-sleeping rat. The Fos protein is stained black and confined to the nucleus. The GAD IRNs are stained brown, and the staining is evident throughout the soma and the proximal dendrites.