Figure 4.

Expression of CREB and phospho-CREB (p-CREB) protein after bilateral zinc sulfate lesion. A–F, CREB and phospho-CREB expression was visualized by immunostaining (red) in PC of control mice (A, D), and 2 d (B, E) and 7 d (C, F) after bilateral zinc sulfate lesion (BZL). G, The density of total nuclei and CREB/phospho-CREB-positive cells per unit volume was stereologically quantified in layers IIa (right) and IIb (left) for CREB (top) and phospho-CREB (bottom). Two and seven days after BZL, phospho-CREB immunoreactivity was markedly reduced in layer IIb (E, F) whereas CREB immunoreactivity was not changed (B, C) compared with the controls (D, A) (**p < 0.01). Data are means + SEM (n = 3). Scale bar, 50 μm.