Table 2.
Mapping of relative Ab-binding epitopes
| Prebound mAb | Competing mAb |
|||||
|---|---|---|---|---|---|---|
| 7F5 | 11E1 | 17D11 | 19E12 | 29D7 | Control mAba | |
| 7F5 | + | +/− | − | − | − | − |
| 11E1 | + | + | − | − | +/− | − |
| 17D11 | − | − | + | +/− | − | − |
| 19E12 | − | − | + | + | − | − |
| 29D7 | − | − | − | − | + | − |
| Control mAba | − | − | − | − | − | N/A |
A preformed complex of each mAb (20 μg/ml) with rhTrkB-Fc (150 ng/ml) was added to each well of 96-well plates coated with competing Abs. After a 1 h incubation period, plates were washed with PBS and the amounts of rhTrkB-Fc remaining in the wells were quantified using peroxidase-conjugated goat anti-human IgG. +, Complete competition; +/−, partial competition; −, no competition; N/A, not applicable.
aIsotype-matched mouse mAb.