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Phospho-JNK levels dramatically decreased with SP600125 treatment. a, Representative Western blots of cultures 24 h after plating that were chronically treated with SP600125 (SP 24Hr), treated with SP600125 for 90 min just before lysing (SP 90 min), or untreated (control). b, Graph showing that chronic and acute treatment with SP600125 significantly inhibited phosphorylation of JNK but did not affect total JNK levels (n = 6 for each condition). ∗∗∗p < 0.001. Error bars indicate SEM.
