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Phosphorylation of the JNK substrate ATF-2 is attenuated by SP600125 (SP). a, Western blots demonstrating that ATF-2 was dually phosphorylated (at T51/T53) under basal conditions, which decreased with acute or chronic inhibition of JNK. CTL, Control. b, Quantification revealed JNK inhibition significantly decreased ATF-2 phosphorylation (n = 6 for each condition). ∗∗∗p < 0.001. Error bars indicate SEM.
