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Serotonergic neurons do not contain Phox2b. Each panel is the superposition of tryptophan hydroxylase immunoreactivity (Alexa 488; green fluorescence) and Phox2b immunoreactivity (Cy3; red fluorescence). A, Parapyramidal area. B, Raphe pallidus. C, RTN. Phox2b-ir nuclei are present in the RTN but absent from all serotonergic neurons. Red immunofluorescence in A and B is an edge artifact at the ventral medullary surface. Scale bar: (in A) A–C, 100 μm.
