Skip to main content
. 2006 Oct 4;26(40):10164–10176. doi: 10.1523/JNEUROSCI.2379-06.2006

Figure 1.

Figure 1.

PSD-95 is expressed and is mostly nonsynaptic in hippocampal neurons at 10 DIV. A, Hippocampal neurons were grown for the indicated times and extracts were subjected to SDS-PAGE and Western blotting with an antibody to PSD-95. B, Neurons cultured for 12 DIV were double-labeled for PSD-95 and synaptophysin. Apposition of stained clusters was quantitated. Only 37.4 + 1.6% of PSD-95 clusters was synaptic (n = 36 neurons). C, Neurons cultured for 17 DIV were double-labeled for PSD-95 and synaptophysin. At this time point, a majority of dendritic PSD-95 clusters colocalize with synaptophysin (arrowheads), whereas most somatic PSD-95 clusters are smaller in size and do not colocalize with synaptophysin (arrows, inset). A minority of somatic PSD-95 clusters colocalize with synaptophysin (arrowheads, inset). Scale bars: B, C, 10 μm, inset, 3.5 μm.