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. 2006 Oct 4;26(40):10164–10176. doi: 10.1523/JNEUROSCI.2379-06.2006

Figure 5.

Figure 5.

PSD-95 inhibition of branching is activity-independent. A, Hippocampal neurons were transfected with cDNAs encoding GFP by itself (vector) or with PSD-95-dsRed1 (PSD-95) at 10 DIV and were treated with either vehicle, 50 μm APV, 10 μm CNQX, or 1 μm TTX for 24 h. Dendrite number was assessed at 12 DIV. B, APV treatment but not PSD-95 overexpression decreases primary dendrite number. Both PSD-95 and APV decreased secondary dendrite number, and the effect is larger when both treatments are performed. n values are as follows: GFP, 10; GFP + APV, 10; PSD-95, 10; PSD-95 + APV, 14. **p < 0.01; ***p < 0.001 by ANOVA with Tukey–Kramer test for correction for multiple comparisons. C, Neither CNQX treatment nor PSD-95 overexpression changes the number of primary dendrites. Both conditions decrease the number of secondary dendrites. n values are as follows: GFP, 30; GFP + CNQX, 28; PSD-95, 27; PSD-95 + CNQX, 22. *p < 0.05; **p < 0.01; ***p < 0.001 by ANOVA with Tukey–Kramer test for correction for multiple comparisons. D, Neither TTX treatment nor PSD-95 overexpression changes the number of primary dendrites. n values are as follows: GFP, 30; GFP + TTX, 26; PSD-95, 27; PSD-95 + TTX, 17. *p < 0.05; **p < 0.01 by ANOVA with Tukey–Kramer test for correction for multiple comparisons. E, Sholl analysis shows that none of the treatments (APV, CNQX, TTX) block PSD-95 effects on dendrite branching. In fact, the effects are likely additive with PSD-95 overexpression proximal to the cell body. F, Proximal Sholl analysis (from E) within the first 63 μm of the soma. Left, APV does not block the effect of PSD-95 (p > 0.38 for all distances, testing for block or cancellation of the effect of PSD-95 by APV + 95). Middle, CNQX does not block the effect of PSD-95 (p > 0.99 for all distances, testing for block or cancellation of the effect of PSD-95 by CNQX + 95). Right, TTX does not block the effect of PSD-95 (p > 0.99 testing for block or cancellation of the effect of PSD-95 by TTX). All analyses were done using Poisson GLMM and ANODEV. Error bars indicate SE.