Figure 1.

Aβ increased the phosphorylation of cPLA₂ and AA release. A, Western blot analysis shows an increase in p-cPLA₂ but not cPLA₂ in astrocytes after treatment with Aβ_1–42 (5 μm) for 30 min. After immunohistochemical development with p-cPLA₂ antibody, the membrane was stripped and reblotted with antibody for total cPLA₂. For testing effects of inhibitors, astrocytes were treated with the ERK inhibitor U0126 (5 μm), the p38 MAPK inhibitor SB203580 (5 μm), and the NADPH oxidase inhibitor apocynin (1 mm) for 30 min before exposure to Aβ_1–42 for 30 min. B, Confocal immunofluorescence images of p-cPLA₂ in astrocytes before and after treatment with Aβ_1–42 (5 μm) for 30 min. Scale bar, 15 μm. C, Aβ_1–42 induced AA release in astrocytes.