Table 1.

Results of SDA verification in Series 2

Surgery performed	SDA	SHsda	Intentionally Incomplete SDA
Verified	15	18	0
Failed verification	14		4
Criteria failed
CCK	4 (1)
HRP	6 (1)		4
Flourogold	11 (5)		4

Data are number of rats failing each criterion, with number of rats failing only that criterion given in parentheses (7 rats failed two criteria; no rat failed all three). The CCK exclusion criterion was that intraperitoneal injection of 4 μg/kg CCK-8 inhibits food intake >30%; HRP criterion was based on anterograde labeling of the NTS; fluorogold criterion was based on retrograde labeling of the DMX (see Materials and Methods for details). The CCK test was not done in intentionally incomplete SDA rats.