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. 2006 Oct 11;26(41):10461–10471. doi: 10.1523/JNEUROSCI.2579-06.2006

Figure 3.

Figure 3.

Transcription and translation are required for the increase in glutamate uptake during L-LTP. A, A translation inhibitor, anisomycin (40 μm), or a transcription inhibitor, DRB (100 μm), was applied from 20 min before HFS to 180 min after HFS. RNA synthesis (DRB) or protein synthesis (anisomycin) inhibitors did not block the appearance of E-LTP but did block the appearance of L-LTP. Data for HFS alone (open boxes) were reproduced from Figure 1B. B, Anisomycin (40 μm) had no effect on the increase in glutamate uptake during E-LTP (30 min after HFS). Data for B were taken from Levenson et al. (2002). C, In contrast to 30 min after HFS, anisomycin (40 μm) or DRB (100 μm) blocked the increase in glutamate uptake 180 min after HFS. Anisomycin or DRB given alone for 180 min had no effect on the basal level of glutamate uptake (data not shown). Data were analyzed using a one-way ANOVA (F(2,12) = 6.7, p < 0.05), and post hoc analysis was done by Tukey–Kramer's test. *Statistically different, p < 0.05. Error bars indicate SEM. Aniso, Anisomycin.