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. 2006 Oct 11;26(41):10461–10471. doi: 10.1523/JNEUROSCI.2579-06.2006

Figure 5.

Figure 5.

Elevating cAMP induced an increase in glutamate uptake during L-LTP. A, Forskolin (50 μm) was applied for 15 min after 20 min baseline recording. L-LTP was observed 180 min after treatment in slices that received test stimulation during forskolin treatment (n = 7; closed diamonds). Synaptic stimulation has been shown before to be necessary for forskolin to elicit L-LTP (Otmakhov et al., 2004). Slices that received forskolin treatment and no test stimulation, showed a transient potentiation at 60 min but did not exhibit L-LTP at 180 min (n = 7; dashed line). Test stimulation by itself (open diamonds) did not induce E-LTP or L-LTP (n = 5). B, Treatment with forskolin or Sp-cAMPs plus test stimulation induced a significant increase in glutamate uptake during L-LTP. Forskolin or test stimulation alone did not induce an increase in glutamate uptake 180 min after treatment. Data were analyzed using a one-way ANOVA (F(3,21) = 5.1; p < 0.01), and post hoc analysis was done by Tukey–Kramer's test. C, Although forskolin alone or forskolin plus test stimulation induced E-LTP (A), glutamate uptake was not changed 30 min after forskolin or forskolin plus test stimulation treatments. *Statistically different, p < 0.05. Error bars indicate SEM. Forsk, Forskolin; Stim, test stimulation.