Figure 1.

NG detection of [Zn²⁺]_i increase during OGD and reperfusion. A, Images of the CA1 region of a hippocampal slice preloaded with NG diacetate ester as described in Materials and Methods. The fluorescence intensities of CA1 pyramidal cells were examined for at least 10 min before (1), during (2), and after (3) perfusion with oxygen–glucose-deprived ACSF bubbled with 95% N₂/5% CO₂. The oxygen–glucose-deprived insult was administered for 30 min, and the slice was reperfused with normal oxygenated ACSF for 40 min. Images 1′–3′ are pseudocolor images of images 1–3. Scale bars, 100 μm. B, A representative graph shows the measured change of NG fluorescence intensity, indicating increases in intracellular Zn²⁺ during the course of OGD and reperfusion. Arrows show the start of OGD-simulated ischemia and reperfusion, respectively. The y-axis represents the fluorescence emission intensity of the dye/ion complex in arbitrary units.