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Microscopic fluorescence images of rat hippocampal sections stained with PI and NG. Images of pyramidal cells of a hippocampal CA1 section after OGD and reperfusion viewed at 40× are shown. A, PI-stained nuclei red with emission at 630 nm. B, NG-stained cell bodies green with emission at 505 nm. C, Composite overlay fluorescence images showing colocalization of PI nuclei within cell bodies labeled with NG. Scale bars, 20 μm.
